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yhcV [2019-08-28 09:16:38]
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yhcV [2019-08-28 09:16:38]

forespore-specific sporulation protein
locus
BSU_09230
pI
5.01
mw
14.77 kDa
protein length
140 aa Sequence Blast
gene length
423 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    997,175 → 997,597

    The protein

    Paralogous protein(s)

  • [protein|8529D83560E934709B20CCF0B72251A74EC272BB|YlbB]:

    • [SW|Domains]

  • 2 [SW|CBS domain]s (8-64, 72-127)

    • Structure

  • [PDB|4FRY] (protein from ''Burkholderia ambifaria'', 30% identity) [Pubmed|23382856]

    • Additional information

  • The gene is annotated in KEGG as an ortholog of IMP dehydrogenase EC 1.1.1.205. No EC annotation is available in Swiss-Prot/MetaSwiss-Prot/MetaCyc.literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]

    • Expression and Regulation

    Operons

    genes
    [gene|AF3ED8916EA02A560EC8D47E4B1C911E871B9AB0|yhcV]
    description
    [Pubmed|12480901]

    sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190,12480901,16497325], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulation

  • expressed during sporulation in the forespore ([protein|search|SigG]) [Pubmed|15699190,12480901,16497325]
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    additional information

  • the mRNA belongs to the 46 most abundant mRNA species in dormant spores [pubmed|30782632]

    • Biological materials

    Mutant

  • MGNA-B474 (yhcV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1473 NBRP B. subtilis, Japan]
  • BKE09230 (Δ[gene|AF3ED8916EA02A560EC8D47E4B1C911E871B9AB0|yhcV]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCAGCACCCCTTTCA, downstream forward: _UP4_TAATAAGAAAGAGCTTGCAG
  • BKK09230 (Δ[gene|AF3ED8916EA02A560EC8D47E4B1C911E871B9AB0|yhcV]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTCAGCACCCCTTTCA, downstream forward: _UP4_TAATAAGAAAGAGCTTGCAG

    • Expression vectors

  • pGP2930 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • References

  • 15699190,12480901,16497325,23382856,30782632